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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
17/05/2022 |
Actualizado : |
02/12/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
PASSOS, J. R. S.; GUERREIRO, D. D.; OTÁVIO, K. S.; SANTOS-NETO, P. C. DOS; SOUZA-NEVES, M.; CUADRO, F.; NUÑEZ-OLIVERA, R.; CRISPO, M.; BEZERRA, M. J. B.; SILVA, R. F.; LIMA, L. F.; FIGUEIREDO, J. R.; BUSTAMANTE-FILHO, I. C.; MENCHACA, A.; MOURA, A. A. |
Afiliación : |
JOSÉ RENATO S. PASSOS, Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; DENISE D. GUERREIRO, Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; KAMILA S. OTÁVIO, Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; P. C. DOS SANTOS-NETO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; MARCELA SOUZA-NEVES, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; FEDERICO CUADRO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; RICHARD NUÑEZ-OLIVERA, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; MARTINA CRISPO, Unidad de Biotecnología en Animales de Laboratorio, Institut Pasteur de Montevideo, Montevideo, Uruguay; MARIA JÚLIA B. BEZERRA, Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; RENATO F. SILVA, Laboratório de Manipulação de Oócitos e Folículos Ovarianos Pré-antrais - LAMOFOPA - Faculdade de Veterinária, Universidade Estadual do Ceará, Fortaleza, Brazil; LARITZA F. LIMA, Laboratório de Manipulação de Oócitos e Folículos Ovarianos Pré-antrais - LAMOFOPA - Faculdade de Veterinária, Universidade Estadual do Ceará, Fortaleza, Brazil; JOSÉ RICARDO FIGUEIREDO, Laboratório de Manipulação de Oócitos e Folículos Ovarianos Pré-antrais - LAMOFOPA - Faculdade de Veterinária, Universidade Estadual do Ceará, Fortaleza, Brazil; IVAN C. BUSTAMANTE-FILHO, aboratório de Biotecnologia da Reprodução Animal, Programa de Pós-graduação em Biotecnologia, Universidade do Vale do Taquari, Lajeado, Brazil; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; ARLINDO A. MOURA, Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil. |
Título : |
Global proteomic analysis of preimplantational ovine embryos produced in vitro. |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
Reproduction in Domestic Animals, 2022, Volume 57, Issue 7; pages 784-797. doi: https://doi.org/10.1111/rda.14122 |
ISSN : |
0936-6768 |
DOI : |
10.1111/rda.14122 |
Idioma : |
Inglés |
Notas : |
Article history: Received 15 February 2022; Accepted 1 April 2022. -- Funding text - The experiments presently described were conducted at the facilities of the (Fundacion IRAUy, Montevideo, Uruguay) and at the (UBAL) of the , Uruguay. Specially, the authors thank Dr. Rosario Durán and Dr. Alejandro Leyva for kindly assisting us in the proteomic experiment. Finnacial support was provided by Fundacion IRAUy; PRONEX 02/2015 (Programa de Apoio a Núcleos de Excelência Pronex/Funcap/CNPq); the Brazilian Research Council?CNPq (grants # 313160/2017‐1 and 438773/2018‐7); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil. Instituto de Reproducción Animal Uruguay Unidad de Biotecnología en Animales de Laboratorio Institut Pasteur de Montevideo. -- Corresponding author: A. Moura, A.; Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; email:arlindo.moura@gmail.com -- Menchaca, A.; Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; mail:menchaca.alejo@gmail.com |
Contenido : |
ABSTRACT. -The present study was conducted to characterize the major proteome of preimplantation (D6) ovine embryos produced in vitro. COCs were aspirated from antral follicles (2–6 mm), matured and fertilized in vitro and cultured until day six. Proteins were ex- tracted separately from three pools of 45 embryos and separately run in SDS-PAGE. Proteins from each pool were individually subjected to in-gel digestion followed by LC-MS/MS. Three ‘raw files’ and protein lists were produced by Pattern Lab software, but only proteins present in all three lists were used for the bioinformatics analyses. There were 2,262 proteins identified in the 6-day-old ovine
embryos, including al- bumin, zona pellucida glycoprotein 2, 3 and 4, peptidyl arginine deiminase 6, actin cytoplasmic 1, gamma-actin 1, pyruvate kinase, heat shock protein 90 and protein disulfide isomerase, among others. Major biological processes linked to the sheep embryo proteome were translation, protein transport and protein stabilization, and molecular functions, defined as ATP binding, oxygen carrier activity and oxygen bind- ing. There were 42 enriched functional clusters
according to the 2,147 genes (UniProt database). Ten selected clusters with potential association with embryo development included translation, structural constituent of ribosomes, ribosomes, nucleosomes, structural constituent of the cytoskeleton, microtubule-based process, translation initiation factor activity, regulation of translational initiation, cell body and nucleotide biosynthetic process. The most representative KEEG pathways were ribosome, oxida- tive phosphorylation, glutathione metabolism, gap junction, mineral absorption, DNA replication and cGMP-PKG signalling pathway. Analyses of functional clusters clearly showed differences associated
with the proteome of preimplantation (D6) sheep em- bryos generated after in vitro fertilization in comparison with in vivo counterparts (Sanchez et al., 2021; https://doi.org/10.1111/rda.13897), confirming that the quality of in vitro derived blastocysts are unlike those produced in vivo. The present study portrays the first comprehensive overview of the proteome of preimplantational ovine embryos grown in vitro.
© 2022 Wiley-VCH GmbH. MenosABSTRACT. -The present study was conducted to characterize the major proteome of preimplantation (D6) ovine embryos produced in vitro. COCs were aspirated from antral follicles (2–6 mm), matured and fertilized in vitro and cultured until day six. Proteins were ex- tracted separately from three pools of 45 embryos and separately run in SDS-PAGE. Proteins from each pool were individually subjected to in-gel digestion followed by LC-MS/MS. Three ‘raw files’ and protein lists were produced by Pattern Lab software, but only proteins present in all three lists were used for the bioinformatics analyses. There were 2,262 proteins identified in the 6-day-old ovine
embryos, including al- bumin, zona pellucida glycoprotein 2, 3 and 4, peptidyl arginine deiminase 6, actin cytoplasmic 1, gamma-actin 1, pyruvate kinase, heat shock protein 90 and protein disulfide isomerase, among others. Major biological processes linked to the sheep embryo proteome were translation, protein transport and protein stabilization, and molecular functions, defined as ATP binding, oxygen carrier activity and oxygen bind- ing. There were 42 enriched functional clusters
according to the 2,147 genes (UniProt database). Ten selected clusters with potential association with embryo development included translation, structural constituent of ribosomes, ribosomes, nucleosomes, structural constituent of the cytoskeleton, microtubule-based process, translation initiation factor activity, regulation of translational i... Presentar Todo |
Palabras claves : |
Embryo development; In vitro fertilization; Mass spectrometry; Oocyte; Ovine; PLATAFORMA SALUD ANIMAL; Proteins. |
Asunto categoría : |
L10 Genética y mejoramiento animal |
Marc : |
LEADER 04571naa a2200409 a 4500 001 1063149 005 2022-12-02 008 2022 bl uuuu u00u1 u #d 022 $a0936-6768 024 7 $a10.1111/rda.14122$2DOI 100 1 $aPASSOS, J. R. S. 245 $aGlobal proteomic analysis of preimplantational ovine embryos produced in vitro.$h[electronic resource] 260 $c2022 500 $aArticle history: Received 15 February 2022; Accepted 1 April 2022. -- Funding text - The experiments presently described were conducted at the facilities of the (Fundacion IRAUy, Montevideo, Uruguay) and at the (UBAL) of the , Uruguay. Specially, the authors thank Dr. Rosario Durán and Dr. Alejandro Leyva for kindly assisting us in the proteomic experiment. Finnacial support was provided by Fundacion IRAUy; PRONEX 02/2015 (Programa de Apoio a Núcleos de Excelência Pronex/Funcap/CNPq); the Brazilian Research Council?CNPq (grants # 313160/2017‐1 and 438773/2018‐7); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil. Instituto de Reproducción Animal Uruguay Unidad de Biotecnología en Animales de Laboratorio Institut Pasteur de Montevideo. -- Corresponding author: A. Moura, A.; Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; email:arlindo.moura@gmail.com -- Menchaca, A.; Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; mail:menchaca.alejo@gmail.com 520 $aABSTRACT. -The present study was conducted to characterize the major proteome of preimplantation (D6) ovine embryos produced in vitro. COCs were aspirated from antral follicles (2–6 mm), matured and fertilized in vitro and cultured until day six. Proteins were ex- tracted separately from three pools of 45 embryos and separately run in SDS-PAGE. Proteins from each pool were individually subjected to in-gel digestion followed by LC-MS/MS. Three ‘raw files’ and protein lists were produced by Pattern Lab software, but only proteins present in all three lists were used for the bioinformatics analyses. There were 2,262 proteins identified in the 6-day-old ovine embryos, including al- bumin, zona pellucida glycoprotein 2, 3 and 4, peptidyl arginine deiminase 6, actin cytoplasmic 1, gamma-actin 1, pyruvate kinase, heat shock protein 90 and protein disulfide isomerase, among others. Major biological processes linked to the sheep embryo proteome were translation, protein transport and protein stabilization, and molecular functions, defined as ATP binding, oxygen carrier activity and oxygen bind- ing. There were 42 enriched functional clusters according to the 2,147 genes (UniProt database). Ten selected clusters with potential association with embryo development included translation, structural constituent of ribosomes, ribosomes, nucleosomes, structural constituent of the cytoskeleton, microtubule-based process, translation initiation factor activity, regulation of translational initiation, cell body and nucleotide biosynthetic process. The most representative KEEG pathways were ribosome, oxida- tive phosphorylation, glutathione metabolism, gap junction, mineral absorption, DNA replication and cGMP-PKG signalling pathway. Analyses of functional clusters clearly showed differences associated with the proteome of preimplantation (D6) sheep em- bryos generated after in vitro fertilization in comparison with in vivo counterparts (Sanchez et al., 2021; https://doi.org/10.1111/rda.13897), confirming that the quality of in vitro derived blastocysts are unlike those produced in vivo. The present study portrays the first comprehensive overview of the proteome of preimplantational ovine embryos grown in vitro. © 2022 Wiley-VCH GmbH. 653 $aEmbryo development 653 $aIn vitro fertilization 653 $aMass spectrometry 653 $aOocyte 653 $aOvine 653 $aPLATAFORMA SALUD ANIMAL 653 $aProteins 700 1 $aGUERREIRO, D. D. 700 1 $aOTÁVIO, K. S. 700 1 $aSANTOS-NETO, P. C. DOS 700 1 $aSOUZA-NEVES, M. 700 1 $aCUADRO, F. 700 1 $aNUÑEZ-OLIVERA, R. 700 1 $aCRISPO, M. 700 1 $aBEZERRA, M. J. B. 700 1 $aSILVA, R. F. 700 1 $aLIMA, L. F. 700 1 $aFIGUEIREDO, J. R. 700 1 $aBUSTAMANTE-FILHO, I. C. 700 1 $aMENCHACA, A. 700 1 $aMOURA, A. A. 773 $tReproduction in Domestic Animals, 2022, Volume 57, Issue 7; pages 784-797. doi: https://doi.org/10.1111/rda.14122
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1. | | MACHADO, M.; CASTRO, M.B.; WILSON, T. M.; GONÇALVES , A. A. B.; PORTIANSKY, E.; RIET-CORREA, F.; BARROS, S. S. Poisoning by Nierembergia veitchii: Effects on vascular smooth muscle cells in the pathogenesis of enzootic calcinosis. Veterinary Pathology, 2022, Volume 59, Issue 5, pages 814-823. doi: https://doi.org/10.1177/03009858221098430 Article history: Article first published online May 19, 2022; Published online September 2022. -- Corresponding Author: Franklin Riet-Correa, Postgraduate Program in Animal Science in the Tropics, School of Veterinary Medicine and Animal...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
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2. | | MACHADO, M.; QUEIROZ, CINTIA R.R.; WILSON, T. M.; SOUSA, D.E.R.; SOTO-BLANCO, B.; ARMIÉN, A.G.; BARROS, S.S.; RIET-CORREA, F.; CASTRO, M.B. Neurological and gastrointestinal manifestations of spontaneous poisoning by Ricinus communis in goats. Toxicon, July 2022, vol. 214, pages 74-77. doi: https://doi.org/10.1016/j.toxicon.2022.05.004 Article history: Received 20 April 2022; Received in revised form 4 May 2022; Accepted 13 May 2022; Available online 20 May 2022.
Corresponding author: Machado, M.; Instituto Nacional de Investigación Agropecuaria, Plataforma de Salud...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
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3. | | MACHADO, M.; QUEIROZ C.R.R.; WILSON T.M.; SOUSA D.E.R.; CASTRO M.B.; SARAVIA, A.; LEE S.T.; ARMIEN A.G.; BARROS S.S.; RIET-CORREA, F. Endemic Xanthium strumarium poisoning in cattle in flooded areas of the Araguari River, Minas Gerais, Brazil. Toxicon, 2021, volume 200, pages 23-29. Doi: https://doi.org/10.1016/j.toxicon.2021.06.019 Article history: Received 22 May 2021; Received in revised form 29 June 2021; Accepted 30 June 2021; Available online 2 July 2021.
Corresponding author: Riet-Correa, F.; Graduate Program in Animal Science in the Tropics, Escola de...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
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4. | | MACHADO, M; WILSON, T.M.; RIBEIRO DE SOUSA, D.E.; LOPES CÂMARA , A.C.; FURLAN, F.H.; SILVA ALMEIDA E MACÊDO, J.T; PUPIN, R.C.; AMARAL DE LEMOS, R.A; ARMIÉN, A.G.; BARROS, S.S.; RIET-CORREA, F.; BOTELHO DE CASTRO, M. Fatal lancehead pit viper (Bothrops spp.) envenomation in horses. Toxicon, December 2019, Volume 170, Pages 41-50. Article history: Received 9 May 2019/ Revised 16 July 2019/ Accepted 1 September 2019/ Available online 6 September 2019.Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
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